phosphorylated stat3 pstat3 duoset ic human phospho stat3  (R&D Systems)

Journal: ACS Omega

Article Title: Biophysical and Biochemical Regulation of Cell Dynamics in Magnetically Assembled Cellular Structures

doi: 10.1021/acsomega.3c02052

Figure Lengend Snippet: Phosphorylated STAT3 (pSTAT3) levels in HBEC3 KT cell lysates measured using an enzyme-linked immunosorbent assay (ELISA), with and without Gadavist exposure ( N = 4). The cells are stimulated with OSM and IL6 for 12 h before the pSTAT3 detection. The statistically significant results are shown with asterisks. The signaling molecule concentrations in ng mL –1 are indicated in parentheses.

Article Snippet: After 24 h incubation, the cells were exposed to either 25 mM Gadavist or regular KSFM (control) for 3 h. Subsequently, the wells were rinsed, and cells were exposed to recombinant human OSM (0.1 and 1 ng mL –1 ; R&D Systems) and IL6 (0.1 and 1 ng mL –1 ; R&D Systems) for 12 h. The pSTAT3 levels were measured in the HBEC3 KT lysates using the pSTAT3 (Y705) DuoSet ELISA kit (R&D Systems), as per the manufacturer’s instructions.

Techniques: Enzyme-linked Immunosorbent Assay